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Recent Publications (2000 - Present)
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(* indicates student co-author.)
"A Redshifted Codon-Optimized Firefly Luciferase is a Sensitive Reporter for Bioluminescence Imaging," H. Caysa, R. Jacob, N. Müther, B. Branchini, M. Messerle and A. Söling, Photochemical and Photobiological Sciences, in press, 2008.
"Spectral-Resolved Gene Technology for Multiplexed Bioluminescence and High-Content Screening," E. Michelini, L. Cevenini, L. Mezzanotte, D. Ablamsky, T. Southworth, B. Branchini and A. Roda, Analytical Chemistry 80: 260-267 (2008).
"Combining Intracellular and secreted bioluminescent reporter proteins for multicolor cell-based assays," E. Michelini, L. Cevenini, L. Mezzanotte, D. Ablamsky, T. Southworth, B. R. Branchini and A. Roda, Photochem Photobiol Sci. 7: 212-217 (2008).
"Synergistic Mutations Produce Blue-Shifted Bioluminescence in Firefly Luciferase," B. R. Branchini, D. M. Ablamsky, J. M. Rosenman*, L. Uzasci*, T. L. Southworth and M. Zimmer, Biochemistry 46 (48): 13847-13855 (2007).
"Development of a Multiplexed Bioluminescent Cell-based Assay wit the Luc Gene from Luciola italica for High Throughput Screening of Cholesterol-Lowering Drugs." E. Michelini, T. L. Southworth, D. Ablamsky, B. R. Branchini and A. Roda, in Proceedings of the 14th International Symposium on Bioluminescence and Chemiluminescence: Chemistry Biology and Applications, San Diego, CA, October 15-19, 2006, edited by A. A. Szalay, P. J. Hill, L. J. Kricka and P. E. Stanley, World Scientific Publishing Co. Pte. Ltd., Singapore, pp. 119-122, 2007.
"Thermostable red and green light-producing firefly luciferase mutants for bioluminescent reporter applications," B.R. Branchini, D.M. Ablamsky, M.H. Murtiashaw, L. Uzasci*, H. Fraga and T.L. Southworth, Analytical Biochemistry, 361 (2): 253-262 (2007).
"Lucifease from the Italian firefly Luciola italica: Molecular cloning and expression," B.R. Branchini, T.L. Southworth, J.P. DeAngelis*, A. Roda, and E. Michelini, in Comparative Biochemistry and Physiology, Part B, 145, pp.159-167 (2006).
"NMR Assignment of the Backbone Resonances of the Firefly Luciferase C-Terminal 14.3 kDa Domain," B.R. Branchini, S.A. Gonzales, and R. Magyar in Journal of Biomolecular NMR, vol 33, p. 73 (2005).
"Red- and Green-Emitting Firefly Lucifease Mutants for Bioluminescent Reporter Applications," B.R. Branchini, T.L. Southworth, N.K. Khattak*, E. Michelini, and A. Roda in Analytical Biochemistry, vol. 345, pp.140-148 (2005).
"Synthesis of an N-Acyl Sulfamate Analog of Luciferyl-AMP: A Stable and Potent Inhibitor of Firefly Luciferase," B.R. Branchini, M.H. Murtiashaw, J.N. Camody, E.E. Mygatt* and T.L. Southworth, in Bioorganic and Medicinal Chemistry Letters, Vol. 15, pp. 3860-3864 (2005).
" An Alternative Mechanism of Bioluminescence Color Determination in Firefly Luciferase," B.R. Branchini, T.L. Southworth, M.H. Murtiashaw, R.M. Magyar, S.A. Gonzalez, M.C. Ruggiero*, and J.G. Stroh, Biochemistry, Vol. 43: 7255-7262 (2004).
"Rational and Random Mutagenesis of Firefly Luciferase to Identify an Efficient Emitter of Red Bioluminescence." B.R. Branchini, T.L. Southworth, N.K. Khattak*, M.H. Murtiashaw, and S.E. Fleet*. In Proceedings of the International Society for Optical Engineering (SPIE), December 2003,Vol. 5329, pp. 170-177 (2004).
"A Mutagenesis Study of the Putative Luciferin Binding Site Residues of Firefly Luciferase," B.R. Branchini, T.L. Southworth, M.H. Murtiashaw, H. Boije, and S.E. Fleet*, Biochemistry, Vol. 42 (35): 10429-10436 (2003).
"Yellow-Green and Red Firefly Bioluminescence from 5,5-Dimethyloxyluciferin," B.R. Branchini, M.H. Murtiashaw, R.A. Magyar, N.C. Portier*, M.C. Ruggiero* and J.G. Stroh, Journal of the American Chemical Society, Vol. 125 (10): 2112-2113 (2002).
"The Role of Active Site Residue Arginine 218 in Firefly Luciferase Bioluminescence," B.R. Branchini, R.A. Magyar, M.H. Murtiashaw and N.C. Portier*, Biochemistry, 40 (8), 2410-2418 (2001).
"The Role of Lysine 529, a Conserved Residue of the Acyl-Adenylate-Forming Enzyme Superfamily, in Firefly Luciferase," B.R. Branchini, M.H. Murtiashaw, R.A. Maygar and S.M. Anderson*, Biochemistry 39 (18), 5433-5440 (2000).