Publications in the last 13 years

(* indicates student coauthor)

  1. "Red-emitting chimeric firefly luciferase for imaging in low ATP cellular environments," Bruce R. Branchini, Tara L. Southworth, Danielle M. Fontaine, Dawn Kohrt, Franceine S. Welcome*, Catherine M. Florentine*, Emma R. Henricks*, Demetria B. DeBartolo, Elisa Michelini, Luca Cevenini, Aldo Roda, Martha J. Grossel, Analytical Biochemistry, Volume 534, 2017, Pages 36-39, ISSN 0003-2697

  2. “Cloning of the Orange Light-Producing Luciferase from Photinus scintillansA New Proposal on how Bioluminescence Color is Determined,” B.R. Branchini, T.L. Southworth, D.M. Fontaine, M.H. Murtiashaw, A. McGurk, M.H. Talukder*, R. Qureshi, D. Yetil, J.A. Sundlov, and A.M. Gulick, Photochem. Photobiol. 93, 479-485 (2017).

  3. “Cloning of the Blue Ghost (Phausis reticulata) Luciferase Reveals a Glowing Source of Green Light,” B.R. Branchini, T.L. Southworth, L.J. Salituro* , D.M. Fontaine, and Y. Oba, Photochem. Photobiol. 93, 473-478 (2017).

  4. “Bioluminescence Imaging of Spheroids for High-throughput Longitudinal Studies on 3D Cell Culture Models,”L. Cevenini, M.M. Calabretta, A. Lopreside, B.R. Branchini, T.L. Southworth, E. Michelini, and A. Roda, Photochem. Photobiol. 93, 531-535 (2017).

  5. “A Highly Sensitive Biosensor for ATP Using a Chimeric Firefly Luciferase,” B.R. Branchini, and T.L. Southworth, Methods Enzymol. 589, 351-364 (2017).

  6. "Firefly Luciferase-Based Sequential Bioluminescence Resonance Energy Transfer (BRET)-Fluorescence Resonance Energy Transfer (FRET) Protease Assay," B. Branchini. In Sung-Bae Kim (ed.), Bioluminescence: Methods and Protocols, Methods in Molecular Biology, vol. 1461, 2016. DOI 10.1007/978-1-4939-3813-1_8

  7. "Probing Bioluminescence Resonance Energy Transfer in Quantum Rod – Luciferase Nanoconjugates," R. Alam, L. Karam, T. L. Doane, K. Coopersmith, D. M. Fontaine, B. R. Branchini, and M. Maye, ACS Nano 10 1969 -1977 (2016).

  8. "Detection of Nitric Oxide Production in Cell Cultures by Luciferin-Luciferase Chemiluminescence," Y. Y. Woldman, T. D. Eubank, A. J. Mock, N. C. Stevens, S. Varadharaj, J. Turco, M. A. Gavrilin, B. R. Branchini, and V. V. Khramtsov, Biochemical and Biophysical Research Communications, 465, 232-238 (2015).

  9. "Experimental Support for a Single Electron-Transfer Oxidation Mechanism in Firefly Bioluminescence, " Bruce R. Branchini, Curran E. Behney, Tara L. Southworth, Danielle M. Fontaine, Andrew M. Gulick, David J. Vinyard and Gary W. Brudvig, Journal of the American Chemical Society (Communication) 137; 7592-7595 (2015), DOI:

  10. "An enhanced chimeric firefly luciferase-inspired enzyme for ATP detection and bioluminescence reporter and imaging applications, " Bruce R. Branchini, Tara L. Southworth, Danielle M. Fontaine, Dawn Kohrt, Munya Talukder*, Elisa Michelini, Luca Cevenini, Aldo Roda and Martha J. Grossel, Analytical Biochemistry 484; 148-153 (2015).

  11. "Multicolor Bioluminescence Boosts Malaria Research: Quantitative Dual-Color Assay and Single-Cell Imaging in Plasmodium falciparum Parasites," L. Cevenini, G. Camarda, E. Michelini, G. Siciliano, M. M. Calabretta, R. Bona, T. R. Santha Kumar, A. Cara, B. R. Branchini, D. A. Fidock, A. Roda, and P. Alano, Analytical Biochemistry 86, 8814-8821 (2014).

  12. "A Photinus pyralis and Luciola italic Chimeric Firefly Luciferase Produces Enhanced Bioluminescence," B. R. Branchini, T. L. Southworth, D. M. Fontaine, A. L. Davis, C. E. Behney and M. H. Murtiashaw, Biochemistry (Rapid Report) 53, 6287-6289 (2014).

  13. "Near Infrared Bioluminescence Resonance Energy Transfer from Firefly Luciferase − Quantum Dot Bionanoconjugates," R. Alam, L. Karam, T. Doane, J. Zylstra, D. Fontaine, B. Branchini, and M. Mathew, Nanotechnology 25 (49): 495606 (7 pp), 2014.

  14. "Multicolor Bioluminescence Boosts Malaria Research: Quantitative Dual-Color Assay and Single-Cell Imaging in Plasmodium falciparum Parasites," L. Cevenini, G. Camarda, E. Michelini, G. Siciliano, M. M. Calabretta, R. Bona, T. R. Santha Kumar, A. Cara, B. R. Branchini, D. A. Fidock, A. Roda, and P. Alano, Analytical Biochemistry 86, 8814-8821 (2014).

  15. "Chemical Analysis of the Luminous Slime Secreted by the Marine Worm Chaetopterus (Annelida, Polychaeta)," B. R. Branchini, C. E. Behney, T. L. Southworth, R. Rawat, D. D. Deheyn, Photochem. Photobiol. 90: 247-251 (2014).

  16. "Novel Multistep BRET-FRET Energy Transfer Using Nanoconjugates of Firefly Proteins, Quantum Dots, and Red Fluorescent Proteins," R. Alam, J. Zylstra, D. M. Fontaine, B. R. Branchini and M. M. Maye, Nanoscale 5 (12): 5303-5306 (2013).etro H

  17. "Firefly Bioluminescence," B. Branchini, in Bioluminescence (edited by. J. Lee). Photobiological Sciences Online (2004, revised in 2013) edited by. D. Valenzeno. Website:

  18. "Novel Heterocyclic Analogues of Firefly Luciferin," C. C. Woodroofe, P. L. Meisenheimer, D. H. Klaubert, Y. Kovic*, J. C. Rosenberg, C. E. Behney, T. L. Southworth and B. R. Branchini, Biochemistry 51: 9807-9813 (2012).

  19. "Crystal Structure of Firefly Luciferase in a Second Catalytic Conformation Supports a Domain Alternation Mechanism," J. A. Sundlov, D. M. Fontaine, T. L. Southworth, B. R. Branchini, and A. M. Gulick, Biochemistry 51: 6493–6495 (2012).

  20. "Designing Quantum Rods for Optimized Energy Transfer with Firefly Luciferase Enzymes, R. Alam, D. Fontaine, B. Branchini and M. Maye, Nano Letters 12: 3251-3256 (2012).

  21. "Bioluminescence Is Produced from a Trapped Firefly Luciferase Conformation Predicted by the Domain Alternation Mechanism," B. R. Branchini, J. C. Rosenberg, D. M. Fontaine, T. L. Southworth, C. E. Behney, and L. Uzasci*, Journal of the American Chemical Society 133: 11088-11091 (2011).

  22. "Sequential Bioluminescence Resonance Energy-Transfer-Fluorescence Resonance EnergyTransfer-Based Ratiometric Protease Assays with Fusion Proteins of Firefly Luciferase and Red Fluorescent Protein," B. R. Branchini, J. C. Rosenberg, D. M. Ablamsky, K. P. Taylor*, T. L. Southworth and S. J. Linder*, Analytical Biochemistry 414: 239-245 (2011).

  23. "Sensitive Dual-Color In Vivo Bioluminescence Imaging Using a New Red Codon Optimized Firefly Luciferase and a Green Click Beetle Luciferase," L Messanotte, I. Que, E. Kaijzel, B. Branchini, A. Roda and C. Lowik, PLoS ONE ( 6 (4): e192777 (2011).

  24. "A Portable Bioluminescence Engineered CellBased Biosensor for OnSite Applications," A. Roda, L. Cevenini E. Michelini and B. R. Branchini, Biosensors and Bioelectronics 26: 3647-3653 (2011).

  25. "Isolated Luciferase Gene of L. italica," Bruce R. Branchini, Tara L. Southworth, Jennifer P. DeAngelis*, Aldo Roda and Elisa Michelini, US Patent number 7,807,429 B2, October 5, 2010.

  26. "Isolated Luciferase Gene of L. italica," Bruce R. Branchini, Tara L. Southworth, Jennifer P. DeAngelis*, Aldo Roda and Elisa Michelini, European Patent number EP 2 002 007 B1, September 29, 2010.

  27. "Biotechnological Improvements of Bioluminescent Systems," K. T. Hamorsky, E. Dikici, C. M. Ensor, S. Daunert, A. L. Davis* and B. R. Branchini. In Chemiluminescence and Bioluminescence Past, Present and Future, edited by A. Roda, RSC Publishing, pp. 443-487, 2010 (ISBN 978-1-84755-812-1).

  28. "Chemically Modified Firefly Luciferase Is an Efficient Source of Near Infrared Light," B. R. Branchini, D. M. Ablamsky, and J. C. Rosenberg, Bioconjugate Chemistry 21 (11): 2023-2030 (2010).

  29. "In Vivo Bioluminescence Imaging for the Study of Intestinal Colonization by Escherichia coli in Mice," M.-L. Foucault, L. Thomas, S. Goussard, B. R. Branchini, and C. Grillot-Courvalin, Applied and Environmental Microbiology 76 (1): 264-274 (2010).

  30. "In Vivo Bioluminescence Imaging of Murine Xenograft Cancer Models with a Red-shifted Thermostable Luciferase," L. Mezzanotte, R. Fazzina, E. Michelini, R. Tonelli, A. Pession, B. Branchini and A. Roda, Molecular Imaging and Biology 12 (4): 406-414 (2010).

  31. "Red-Emitting Luciferases for Bioluminescence Reporter and Imaging Applications," B. R. Branchini, D. M. Ablamsky, A. L. Davis*, T. L. Southworth, B. Butler, F. Fan, A. P. Jathoul and M. A. Pule, Analytical Biochemistry 396 (2): 290-297 (2010).

  32. "The Effects of Elevated Osmotic Concentration on Control of Germination in the Gemmules of Freshwater Sponges Eunapius fragilis and Anheteromeyania ryderi," S. H. Loomis, A. Bettridge and B. R. Branchini, Physiological and Biochemical Zoology 82: 388-395 (2009).

  33. "A Redshifted Codon-Optimized Firefly Luciferase is a Sensitive Reporter for Bioluminescence Imaging," Photochemical and Photobiological Sciences, " H. Caysa, R. Jacob, N. Müther, B. Branchini, M. Messerle and A. Söling, Photochem. Photobiol. Sci. 8: 52-56 (2009).

  34. "Combining Intracellular and Secreted Bioluminescent Reporter Proteins for Multicolor Cell-Based Assays," E. Michelini, L. Cevenini, L. Mezzanotte, D. Ablamsky, T. Southworth, B. R. Branchini and A. Roda, Photochem. Photobiol. Sci. 7: 212-217 (2008).

  35. "Spectral-Resolved Gene Technology for Multiplexed Bioluminescence and High-Content Screening," E. Michelini, L. Cevenini, L. Mezzanotte, D. Ablamsky, T. Southworth, B. Branchini and A. Roda, Anal. Chem. 80: 260- 267 (2008).

  36. "Synergistic Mutations Produce Blue-Shifted Bioluminescence in Firefly Luciferase," B. Branchini, D. Ablamsky, J. Rosenman*, L. Uzasci*, T. Southworth and M. Zimmer, Biochemistry 46: 13847-13855 (2007).

  37. "Development of a Multiplexed Bioluminescent Cell-based Assay wit the Luc Gene from Luciola italica for High Throughput Screening of Cholesterol-Lowering Drugs." E. Michelini, T. L. Southworth, D. Ablamsky, B. R. Branchini and A. Roda, in Proceedings of the 14th International Symposium on Bioluminescence and Chemiluminescence: Chemistry Biology and Applications, San Diego, CA, October 15-19, 2006, edited by A. A. Szalay, P. J. Hill, L. J. Kricka and P. E. Stanley, World Scientific Publishing Co. Pte. Ltd., Singapore, pp. 119-122, 2007.

  38. "Thermostable red and green light-producing firefly luciferase mutants for bioluminescent reporter applications," B. R. Branchini, D. M. Ablamsky, M. H. Murtiashaw, L. Uzasci*, H. Fraga and T.L. Southworth, Analytical Biochemistry 361 (2): 253-262 (2007).

  39. "Luciferase from the Italian Firefly Luciola italica: Molecular Cloning and Expression," B. R. Branchini, T. L. Southworth, J. P. DeAngelis*, A. Roda and E. Michelini, Comparative Biochemistry and Physiology, Part B, 145 (2): 159-167 (2006).

  40. "NMR Assignment of the Backbone Resonances of the Firefly Luciferase C-terminal 14.3 kDa Domain," B. R. Branchini, S. A. Gonzalez and R. A. Magyar, Journal of Biomolecular NMR 33 (1): 73 (2005).

  41. "Red- and Green-Emitting Firefly Luciferase Mutants for Bioluminescent Reporter Applications," B. R. Branchini, T. L. Southworth, N. F. Khattak*, E. Michelini, and A. Roda, Analytical Biochemistry 345: 140-148 (2005).

  42. "Synthesis of an N-Acyl Sulfamate Analogue of Luciferyl-AMP: a Stable and Potent Inhibitor of Firefly Luciferase," B. R. Branchini, M. H. Murtiashaw, J. N. Carmody*, E. E. Mygatt* and T. L. Southworth, Bioorganic and Medicinal Chemistry Letters 15 (17): 3860-3864 (2005).

  43. "Mutagenesis Evidence that the Partial Reactions of Firefly Bioluminescence are Catalyzed by Different Conformations of the Luciferase C-Terminal Domain," B. R. Branchini, T. L. Southworth, M. H. Murtiashaw, S. R. Wilkinson*, N. F. Khattak*, J. C. Rosenberg* and M. Zimmer, Biochemistry 44: 1385-1393 (2005).

  44. "Modules in Emerging Fields, " Vol. 6: Bioanalytical Applications of Bioluminescence, "A. Roda, B. Branchini, M. Zimmer and J. Hayes, Connecticut College Howard Hughes Medical Institute series (CD format), 2004.

  45. "An Alternative Mechanism of Bioluminescence Color Determination in Firefly Luciferase," B. R. Branchini, T. L. Southworth, M. H. Murtiashaw, R. A. Magyar, S. A. Gonzalez, M. C. Ruggiero* and J. G. Stroh, Biochemistry 43, 7255-7262 (2004).

  46. "Rational and Random Mutagenesis of Firefly Luciferase to Identify an Efficient Emitter of Red Bioluminescence, " B. R. Branchini, T. L. Southworth, N. F. Khattak*, M. H. Murtiashaw, and S. E. Fleet*. In Genetically Engineered and Optical Probes for Biomedical Applications II, edited by A. P. Savitsky et al., Proc. SPIE 5329, pp. 170-177, 2004.

  47. "A Mutagenesis Study of the Putative Luciferin Binding Site Residues of Firefly Luciferase," B. R. Branchini, T. L. Southworth, M. H. Murtiashaw, H. Boije and S. E. Fleet*, Biochemistry 42 (35): 10429-10436 (2003).